DL-Protocol for cells that are permeabilized in the O2k chamber (ce-pce): SUIT-007 O2 ce-pce D030.DL-Protocol for cells that are permeabilized in the O2k chamber (ce-pce): SUIT-006 O2 ce-pce D029.DL-Protocol for coupling control with living cells and cell viability module (ce-pce): SUIT-003 O2 ce-pce D020.DL-Protocol for living cells (ce): SUIT-003 O2 ce D009, SUIT-003 O2 ce D037 and SUIT-003 O2 ce D038.DL-Protocol for cells that are permeabilized in the O2k chamber (ce-pce): SUIT-002 O2 ce-pce D007 SUIT-002 O2 ce-pce D007a (for PBMC and PLT). ![]() DL-Protocol for cells that are permeabilized in the O2k chamber (ce-pce): SUIT-001 O2 ce-pce D003 SUIT-001 O2 ce-pce D004 (for PBMC and PLT).ROUTINE respiration ROUTINE respiration can be evaluated in the following SUIT protocols: Guidelines for performing and evaluating respirometric assays: DatLab oxygen flux: performance and data analysis.To reach steady state in ROUTINE respiration may require up to 10-15 min, waiting longer is not recommended.DatLab oxygen flux: performance and data analysis Proton leaks dissipate energy of translocated protons from the positive phase to the negative phase (modified after Gnaiger 2020 BEC MitoPathways). Proton flow to the negative matrix phase (H + in) drives the phosphorylation of ADP to ATP. H + out are protons pumped out of the matrix phase to the positive P-phase. 2 indicates the reduced hydrogen equivalents of CHO substrates and electron transfer to oxygen, with a variety of energy substrates from carbohydrates through glycolysis to the tricarboxylic acid cycle (TCA) and glycerophosphate dehydrogenase complex (C GpDH), amino acid oxidation (AAO) and fatty acid oxidation (FAO through the electron transferring flavoprotein complex, C ETF). Ĭoupled energy cycles and and proton circuit of mitochondrial respiration in living cells controlled by aerobic ATP demand in the ROUTINE state of activity. Reference: Gnaiger 2020 BEC MitoPathways, Gnaiger 2008 POS Contributed by Gnaiger E (last update), edited by Cardoso LHD. R is corrected for residual oxygen consumption (ROX), whereas R´ is the uncorrected apparent ROUTINE respiration or total cellular oxygen consumption of cells including ROX. ![]() R cannot be measured in permeabilized cells or isolated mitochondria. In media without energy substrates, R depends on endogenous substrates. R and growth of cells is supported by exogenous substrates in culture media. If these conditions are defined and remain consistent within a given context, then the simple symbol R for respiratory state can be used to substitute the more explicit expression for respiratory activity. ![]() The conditions for measurement and expression of respiration vary ( oxygen flux in state R, J O 2 R or oxygen flow in state R, I O 2 R). In the living cell, ROUTINE respiration ( R) or ROUTINE activity in the physiological coupling state is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation (intrinsic uncoupling and pathological dyscoupling).
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